Journal: Cells

Article Title: Involvement of Cyclooxygenase-2 in Establishing an Immunosuppressive Microenvironment in Tumorspheres Derived from TMZ-Resistant Glioblastoma Cell Lines and Primary Cultures

doi: 10.3390/cells13030258

Figure Lengend Snippet: List of primary antibodies used in the present study.

Article Snippet: rabbit monoclonal anti-osteopontin , 1:1000 , Boster Biological Technology, Pleasanton, CA, USA.

Techniques:

Journal: Frontiers in Cell and Developmental Biology

Article Title: NF-κB/p65 Competes With Peroxisome Proliferator-Activated Receptor Gamma for Transient Receptor Potential Channel 6 in Hypoxia-Induced Human Pulmonary Arterial Smooth Muscle Cells

doi: 10.3389/fcell.2021.656625

Figure Lengend Snippet: The PCR primer sequences used in this study.

Article Snippet: The specific primary antibodies against p65 (1:1,000, Abcam), p-p65 (1:1,000), PPARγ (1:1,000), p-PPARγ (1:500), TRPC1 (1:2,000), TRPC6 (1:2,000), Bax (1:2,000), Bcl-2 (1:2,000), GAPDH (1:10,000), and Histone H3 (1:500) were used for primary incubation, and anti-rabbit/rat IgG secondary antibodies (1:20,000, Boster Biotechnology, Wuhan, China) were used for secondary incubation.

Techniques:

Journal: Frontiers in Cell and Developmental Biology

Article Title: NF-κB/p65 Competes With Peroxisome Proliferator-Activated Receptor Gamma for Transient Receptor Potential Channel 6 in Hypoxia-Induced Human Pulmonary Arterial Smooth Muscle Cells

doi: 10.3389/fcell.2021.656625

Figure Lengend Snippet: The expression of Bax, Bcl-2, TRPC1, TRPC6, and PPARγ proteins in the hPASMCs. (A,B) The expression of Bax and Bcl-2 proteins in the hPASMCs in response to hypoxia, pcDNA-p65, and shp65 treatments. (C,D) The expression of the TRPC1 and TRPC6 proteins. (E) The fold change of the p-PPARγ to total PPARγ. Each experiment was repeated three times. The differences were analyzed using one-way ANOVA. CTRL, control.

Article Snippet: The specific primary antibodies against p65 (1:1,000, Abcam), p-p65 (1:1,000), PPARγ (1:1,000), p-PPARγ (1:500), TRPC1 (1:2,000), TRPC6 (1:2,000), Bax (1:2,000), Bcl-2 (1:2,000), GAPDH (1:10,000), and Histone H3 (1:500) were used for primary incubation, and anti-rabbit/rat IgG secondary antibodies (1:20,000, Boster Biotechnology, Wuhan, China) were used for secondary incubation.

Techniques: Expressing, Control

Journal: Frontiers in Cell and Developmental Biology

Article Title: NF-κB/p65 Competes With Peroxisome Proliferator-Activated Receptor Gamma for Transient Receptor Potential Channel 6 in Hypoxia-Induced Human Pulmonary Arterial Smooth Muscle Cells

doi: 10.3389/fcell.2021.656625

Figure Lengend Snippet: The prediction and validation of p65 protein binding to the promoter region of TRPC6. (A) The predicted binding sites of the p65 and PPARγ proteins in the promoter regions of the TRPC6 gene. (B) The validation of the binding of the p65 protein on the three sites in the promoter of the TRPC6 gene using the dual-luciferase reporter assay. (C) ChIP confirmed the binding of p65 antibodies to the site 1 and site 3 regions on the promoter region of the TRPC6 gene. (D,E) EMSA for the binding of the p65 antibody on the two sites. The differences were analyzed using one-way ANOVA. CTRL, control.

Article Snippet: The specific primary antibodies against p65 (1:1,000, Abcam), p-p65 (1:1,000), PPARγ (1:1,000), p-PPARγ (1:500), TRPC1 (1:2,000), TRPC6 (1:2,000), Bax (1:2,000), Bcl-2 (1:2,000), GAPDH (1:10,000), and Histone H3 (1:500) were used for primary incubation, and anti-rabbit/rat IgG secondary antibodies (1:20,000, Boster Biotechnology, Wuhan, China) were used for secondary incubation.

Techniques: Biomarker Discovery, Protein Binding, Binding Assay, Luciferase, Reporter Assay, Control

Journal: Frontiers in Cell and Developmental Biology

Article Title: NF-κB/p65 Competes With Peroxisome Proliferator-Activated Receptor Gamma for Transient Receptor Potential Channel 6 in Hypoxia-Induced Human Pulmonary Arterial Smooth Muscle Cells

doi: 10.3389/fcell.2021.656625

Figure Lengend Snippet: The ChIP-PCR analysis for the binding of the p65 and Par proteins in the promoter regions of the TRPC6 gene in response to p65 overexpression or inhibition. (A,B) The PCR results of the DNA fragments including the promoter regions of the TRPC6 gene (site 1 and site 2 in ) enriched by the PPARγ antibody. (C,D) The PCR results of the DNA fragments, including the promoter regions of the TRPC6 gene (site 1 and site 3 in ) enriched by the p65 antibody. Each experiment was repeated three times. The differences were analyzed using one-way ANOVA. CTRL, control.

Article Snippet: The specific primary antibodies against p65 (1:1,000, Abcam), p-p65 (1:1,000), PPARγ (1:1,000), p-PPARγ (1:500), TRPC1 (1:2,000), TRPC6 (1:2,000), Bax (1:2,000), Bcl-2 (1:2,000), GAPDH (1:10,000), and Histone H3 (1:500) were used for primary incubation, and anti-rabbit/rat IgG secondary antibodies (1:20,000, Boster Biotechnology, Wuhan, China) were used for secondary incubation.

Techniques: Binding Assay, Over Expression, Inhibition, Control

Journal: Frontiers in Cell and Developmental Biology

Article Title: NF-κB/p65 Competes With Peroxisome Proliferator-Activated Receptor Gamma for Transient Receptor Potential Channel 6 in Hypoxia-Induced Human Pulmonary Arterial Smooth Muscle Cells

doi: 10.3389/fcell.2021.656625

Figure Lengend Snippet: The ChIP-PCR analysis for the binding of the p65 and PPARγ proteins in the promoter regions of the TRPC6 gene in response to PPARγ overexpression or inhibition. (A,B) The PCR results of the DNA fragments, including the promoter regions of the TRPC6 gene (site 1 and site 2 in ) enriched by the p65 antibody. (C,D) The PCR results of the DNA fragments, including the promoter regions of the TRPC6 gene (site 1 and site 3 in ) enriched by the PPARγ antibody. Each experiment was repeated three times. The differences were analyzed using one-way ANOVA. CTRL, control.

Article Snippet: The specific primary antibodies against p65 (1:1,000, Abcam), p-p65 (1:1,000), PPARγ (1:1,000), p-PPARγ (1:500), TRPC1 (1:2,000), TRPC6 (1:2,000), Bax (1:2,000), Bcl-2 (1:2,000), GAPDH (1:10,000), and Histone H3 (1:500) were used for primary incubation, and anti-rabbit/rat IgG secondary antibodies (1:20,000, Boster Biotechnology, Wuhan, China) were used for secondary incubation.

Techniques: Binding Assay, Over Expression, Inhibition, Control

Journal: Frontiers in Cell and Developmental Biology

Article Title: NF-κB/p65 Competes With Peroxisome Proliferator-Activated Receptor Gamma for Transient Receptor Potential Channel 6 in Hypoxia-Induced Human Pulmonary Arterial Smooth Muscle Cells

doi: 10.3389/fcell.2021.656625

Figure Lengend Snippet: The schematic diagram illustrating the regulation of p65 and PPARγ on TRPC6-mediated SOCE and PAH. hPASMCs, human pulmonary arterial smooth muscle cells; PAH, pulmonary artery hypertension; SOCE, store-operated calcium entry; TRPC, transient receptor potential channel.

Article Snippet: The specific primary antibodies against p65 (1:1,000, Abcam), p-p65 (1:1,000), PPARγ (1:1,000), p-PPARγ (1:500), TRPC1 (1:2,000), TRPC6 (1:2,000), Bax (1:2,000), Bcl-2 (1:2,000), GAPDH (1:10,000), and Histone H3 (1:500) were used for primary incubation, and anti-rabbit/rat IgG secondary antibodies (1:20,000, Boster Biotechnology, Wuhan, China) were used for secondary incubation.

Techniques: